​PCR detection of treponemes in beef cattle digital dermatitis lesions

Sample numberGroup-specific PCR*Treponema genus-specific PCR
DetailsLesion site123
1Gloucestershire Farm 1, animal 1Hind right foot+++
2Gloucestershire Farm 1, animal 2Front left foot++++
3Gloucestershire Farm 2, animal 1Hind right foot++++
4Gloucestershire Farm 2, animal 2Hind right foot+++
  • *Groups 1, 2 and 3 are Treponema medium/T vincentii-like, Treponema phagedenis-like and Treponema pedis spirochaetes, respectively, which are routinely found in BDD lesions