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Investigating the presence of equine piroplasmosis in Ireland
  1. Robert M Coultous1,
  2. Desmond P Leadon2,
  3. Brian R Shiels1,
  4. David Sutton3 and
  5. William Weir3
  1. 1University of Glasgow Institute of Biodiversity, Animal Health and Comparative Medicine, Glasgow, UK
  2. 2Irish Equine Centre, Naas, Ireland
  3. 3University of Glasgow School of Veterinary Medicine, Glasgow, UK
  1. Correspondence to Dr Robert M Coultous; robert.coultous{at}glasgow.ac.uk

Abstract

Background Equine piroplasmosis (EP) is a notifiable disease in Ireland and a significant concern to domestic and international equine industries. Information regarding EP presence in Ireland is currently limited. This retrospective surveillance study describes a serological and molecular analysis of blood samples submitted to the Irish Equine Centre for EP testing between January 2013 and April 2016.

Methods Following serological testing, seropositive samples were screened using a PCR targeting the 18S ribosomal RNA gene. Amplicon sequences were bioinformatically analysed to identify the parasite species and to assess genetic diversity.

Results From 2099 screened equine blood samples, 2.5 per cent and 1 per cent were seropositive for Theileria equi and Babesia caballi, respectively. T equi DNA was detected in 9 per cent of the seropositive samples while B caballi DNA was not detected in any sample. The T equi DNA sequences displayed no genetic diversity at this locus, in contrast to samples from the UK and from endemic areas.

Conclusion Detection of EP-seropositive and parasitaemic horses in Ireland indicates a clear and present health risk to the equine population. It is recommended that owners adopt appropriate biosecurity measures and that clinicians are mindful of this disease as a differential diagnosis.

  • equine piroplasmosis
  • Babesia caballi
  • Theileria equi
  • Ireland
  • genetics
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Footnotes

  • Twitter @RobCoultous

  • Funding The Horserace Betting Levy Board (HBLB) funded the cost of the nested PCR screening and Sanger sequencing as part of an HBLB research scholarship held by RMC (VET/RS/254). RMC is currently supported by an HBLB research fellowship (VET/EPDF/2019-1).

  • Competing interests None declared.

  • Provenance and peer review Not commissioned; externally peer reviewed.

  • Data availability statement Data are available in a public, open access repository. Data may be obtained from a third party and are not publicly available. Data may be obtained from RMC (ORCID https://orcid.org/0000-0002-6905-3915), following approval for use from the Irish Equine Centre.

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