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Use of four-layer porcine small intestinal submucosa alone as a scaffold for the treatment of deep corneal defects in dogs and cats: preliminary results
  1. Laura Barachetti1,2,
  2. Marco Zanni3,
  3. Damiano Stefanello1 and
  4. Antonella Rampazzo4
  1. 1Department of Veterinary Medicine, University of Milan, Milan, Italy
  2. 2Dipartimento di Oftalmologia, Istituto Veterinario di Novara, Granozzo con Monticello, Italy
  3. 3Department of Veterinary Science and Public Health, University of Milan, Milan, Italy
  4. 4Equine Department, Universitat Zurich Vetsuisse-Fakultat, Zurich, Switzerland
  1. Correspondence to Dr Laura Barachetti; laurabarachetti{at}


Background To describe the efficacy of four-layer porcine small intestinal submucosa (Vetrix BioSIS plus+) as single scaffold for the treatment of deep corneal lesions in dogs and cats.

Methods 10 dogs and 3 cats with deep or full thickness corneal defects were treated surgically with BioSIS plus graft. Corneal transparency scores and vision were evaluated.

Results Lesions in dogs were four perforations, three descemetoceles, two limbal melanocytomas and one deep corneal ulcer. In cats, there were one limbal melanocytoma and two perforations. The average length of the follow-up was 86 days. In all, 12 out of 13 eyes treated were visual at last recheck (92.3 per cent). The scars were mild eight cases (66.7 per cent), but denser in four cases (33.4 per cent). Complication were partial collagenolysis in three cases (25 per cent), which resolved with medical therapy, mild corneal pigmentation in one case (8.4 per cent) and anterior synechia in one case (8.4 per cent). One case experienced severe collagenolysis and was enucleated 21 days postoperatively.

Conclusions Four-layer porcine SIS graft was successfully used for surgical treatment of deep corneal lesions in selected corneal diseases in a small series of dogs and cats, with good results in terms of mechanic support and corneal transparency.

  • cornea
  • BioSIS
  • dog
  • cat
  • surgery
  • graft
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  • Funding The authors have not declared a specific grant for this research from any funding agency in the public, commercial or not-for-profit sectors.

  • Competing interests None declared.

  • Provenance and peer review Not commissioned; externally peer reviewed.

  • Data availability statement Data are available upon reasonable request. All data relevant to the study are included in the article or uploaded as supplementary information. Data are available from Laura Barachetti DVM, PhD, Dipl. ECVO e-mail:

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