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Towards European harmonisation of contagious equine metritis diagnosis through interlaboratory trials
  1. Sandrine Petry, PhD1,
  2. Marie-France Breuil, BSc1,
  3. Fabien Duquesne, PhD1 and
  4. Claire Laugier, MVD, PhD2
  1. 1Bacteriology Unit, Dozulé Laboratory for Equine Diseases, ANSES, Dozulé, France
  2. 2Dozulé Laboratory for Equine Diseases, ANSES, Dozulé, France
  1. E-mail for correspondenceDozulé Laboratory for Equine Diseases, Bacteriology Unit, ANSES, Dozulé 14430, France; sandrine.petry{at}anses.fr

Abstract

The performance of culture and PCR methods routinely used to diagnose contagious equine metritis (CEM) was evaluated and compared by two interlaboratory trials involving a total of 24 European laboratories, including 22 National Reference Laboratories for CEM. Samples were swab specimens artificially contaminated with bacteria present in the genital tract of Equidae, some with and some without Taylorella equigenitalis, the causative agent of CEM, and T asinigenitalis, responsible for possible misidentification as T equigenitalis. Throughout both interlaboratory trials, PCR performed better in terms of specificity and sensitivity than the culture method, supporting the assertion that PCR should be accepted for CEM diagnosis. However, the culture performance during the second interlaboratory trial was better than during the first one, suggesting that the expertise of participants improved. This reveals the advantage of regular interlaboratory trials to constantly improve the expertise of laboratories. It also shows the need to develop new culture media that are more selective and/or better geared to the metabolism of T equigenitalis in order to improve the bacteriological diagnosis of CEM.

  • contagious equine metritis
  • taylorella equigenitalis
  • diagnostic tests
  • interlaboratory trial

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Footnotes

  • Funding This work was mostly supported by the European Commission (via the budget of the EURL for equine diseases other than African horse sickness).

  • Competing interests None declared.

  • Provenance and peer review Not commissioned; externally peer reviewed.

  • Collaborators European Laboratories involved in contagious equine metritis.

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