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The A (H1N1) pdm09 influenza pandemic and, most recently, the A (H3N2) variant outbreak in several areas of the USA are examples of swine influenza viruses infecting humans. These cases highlight the need for reliable and rapid diagnostic tests to elucidate the epidemiology and evolution of swine influenza viruses (Smith and others 2009, Centers for Disease Control and Prevention 2012a, b, c). Currently, there are numerous commercial kits based on rapid-immunomigration techniques available for a fast detection of avian influenza viruses (Chen and others 2010). These rapid-immunomigration kits use specific antibodies against nucleoprotein (NP) of type A influenza viruses. Because the NP proteins are highly conserved between influenza viruses (Shu and others 1993, Li and others 2009), it is of relevance to assess if rapid-immunomigration kits designed for avian influenza are effective to detect influenza viruses in swine populations. Thus, the main goal of the present study was to evaluate the sensitivity and specificity of a commercial kit intended for avian samples, for samples obtained from backyard and commercial farm pigs.
All procedures in this study were performed following the Good Laboratory Practices and its recommended biosecurity guidelines (Centers for Disease Control and Prevention 2012a, b, c). Handling and sampling of animals were performed as indicated in the Mexican Official Regulation 062-ZOO-1999, which outlines technical specifications for the reproduction, care and use of laboratory animals …
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