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Pharmacokinetic interactions of marbofloxacin with anti-inflammatory drugs in buffalo calves
  1. E. E. Baroni, DVM, PhD1,
  2. S. Rubio, DVM2,
  3. C. Rodríguez, DVM, PhD2,
  4. J. J. De Lucas, DVM, PhD2,
  5. H. Fernández, DVM1 and
  6. M. S. Andrés, DVM, PhD2
  1. Cátedra de Farmacología y Toxicología, Facultad de Ciencias Veterinarias, Universidad Nacional del Litoral, 3080 Esperanza (Santa Fe), Argentina
  2. Departamento de Toxicología y Farmacología, Facultad de Veterinaria, Universidad Complutense de Madrid, 28040 Madrid, Spain
  1. Correspondence to Dr Rodríguez, e-mail: rodfermc{at}

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ANTIMICROBIAL agents are usually combined with NSAIDs to treat various systemic infections accompanied by fever and other inflammatory conditions. They can also be administered with steroidal anti-inflammatory drugs (SAIDs) to relieve suffering caused by inflammation. A pharmacological interaction between the two types of drug has been described in previous studies (Post and others 2002, 2003, Sidhu and others 2010, Ogino and others 2005).

The combined use of anti-inflammatory and antimicrobial drugs is common clinical practice in young buffaloes due to enteric and respiratory infections, which are a significant problem and usually result in economic losses in nursing calves (Bukhari and others 2010). This short communication aimed to establish, in this species, the serum concentration-time profile and pharmacokinetic parameters of marbofloxacin (MBF), after intramuscular administration alone and in combination with intramuscular administration of ketoprofen (KPF), flunixin meglumine (FM) or dexamethasone (DXM), and to integrate pharmacokinetic/pharmacodynamic (PK/PD) data.

Twenty-four, seven- to 15-day-old clinically healthy buffalo calves (mean [sd] weight 60.43 [6.75] kg) were included in the study and randomly allocated to one of four treatment groups. A parallel design was used, taking into account the age of the selected animals. All groups received a 2 mg/kg dose of MBF intramuscularly in the semitendinous muscle. The first group received only MBF, while the other three groups also received a dose of one of three drugs: 3 mg/kg KPF (MBF+KTF), 2.5 mg/kg FM (MBF+FM) or 0.1 mg/kg DXM (MBF+DXM) intramuscularly in the opposite semitendinous muscle. The study was approved by the Animal Experimentation Ethics Committee at the School of Veterinary Medicine, Universidad Nacional del Litoral, Argentina.

Serum MBF concentrations were quantified using HPLC/ultraviolet (Waxman and others 2001). The quantification limit was 0.025 µg/ml and the method was linear up to 15 µg/ml. The mean (sd) precision …

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