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Thymidine kinase is a biomarker for enzootic bovine leukosis

M. Konishi, S. Kobayashi, T. Tokunaga and others

BMC Veterinary Research (2019)

doi: 10.1186/s12917-019-2158-4

• What did the research find?

The biomarkers with the highest sensitivity and specificity for enzootic bovine leukosis (EBL) were serum thymidine kinase (TK) (97.1 per cent and 97.0 per cent, respectively) and serum lactate dehydrogenase isozyme 2 (LDH2) (82.8 per cent and 81.7 per cent, respectively). The number of peripheral blood lymphocytes and the proviral load of bovine leukaemia virus (BLV) – the causative agent of EBL – had the lowest sensitivity and specificity.

• How was it conducted?

Blood samples collected from 281 cattle with EBL and 198 cattle infected with BLV but that had not developed EBL were evaluated. A biochemical analyser was used to measure the activity of TK, total LDH and LDH isozymes 1–5, and PCR assays were performed to quantify bovine tumour necrosis factor receptor mRNAs and BLV proviral load. In addition, the numbers of leukocytes in each sample were counted. Receiver operating characteristic curve …

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