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Detection of Mycoplasma hyopneumoniae in piglet processing fluids
  1. Carles Vilalta1,
  2. Juan Manuel Sanhueza1,
  3. Deborah Murray2,
  4. Levi Johnson2 and
  5. Maria Pieters1
  1. 1 Veterinary Population Medicine, University of Minnesota, St Paul, Minnesota, USA
  2. 2 New Fashion Pork, Jackson, Minnesota, USA
  1. Correspondence to Dr Carles Vilalta; cvilalta{at}


Processing fluid (PF) is a sample type composed of fluids obtained from testicles and tails as the product of piglet processing. Mycoplasma hyopneumoniae is a bacterium that colonises the respiratory tract of pigs and has rarely been detected in tissues outside the respiratory system. No data exist in the literature regarding detection of M hyopneumoniae in PF or its use for herd monitoring of this pathogen. The main goal of this study was to evaluate the feasibility of detecting M hyopneumoniae in PF. Testicles and tails of 21 conveniently selected litters from a commercial sow farm were collected, by litter, and tested for M hyopneumoniae by real time-PCR. Daily aggregated processing tissues were collected for a two-month period to assess the detection of M hyopneumoniae in PF. The comparison in the percentage of positive samples in fluids from testicles (38 per cent, 8/21) or tails (4.8 per cent, 1/21) was significantly different (P=0.023). The percentage of daily aggregated PF with cycle threshold values up to 37 was 52.9 per cent (9/17) and 26.7 per cent (4/15) for December and January, respectively. Overall, these data show detection of M hyopneumoniae in PF for the first time and points at the potential use of this sample for monitoring of this bacterium in breeding farms.

  • mycoplasma hyopneumoniae
  • processing fluid
  • monitoring
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  • Correction notice The expansion of RT PCR has been amended from reverse transcriptase-PCR to real time-PCR.

  • Funding This study was funded by the Swine Disease Eradication Center of the University of Minnesota.

  • Competing interests None declared.

  • Provenance and peer review Not commissioned; externally peer reviewed.

  • Data availability statement All data relevant to the study are included in the article.

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