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Porcine reproductive and respiratory syndrome virus RNA detection in different matrices under typical storage conditions in the UK
  1. Jinghui Fan1,2,
  2. Priscilla F Gerber3,
  3. Ana Cubas Atienzar1,
  4. Lysan Eppink1,
  5. Chong Wang4 and
  6. Tanja Opriessnig1,4
  1. 1 The Roslin Institute, University of Edinburgh, Midlothian, UK
  2. 2 College of Veterinary Medicine, Agricultural University of Hebei, Baoding, Hebei, China
  3. 3 Animal Science, School of Environmental and Rural Science, University of New England, Armidale, Australia
  4. 4 Department of Veterinary Diagnostic and Production Animal Medicine, Iowa State University, Ames, USA
  1. E-mail for correspondence; tanja.opriessnig{at}roslin.ed.ac.uk, tanjaopr{at}iastate.edu

Abstract

In the UK, approximately 40 per cent of the pig breeding herds are outdoors. To monitor their porcine reproductive and respiratory syndrome virus (PRRSV) status, blood is collected commonly from piglets around weaning. Sample collection in British outdoor pigs often occurs during the early morning hours when the piglets tend to accumulate inside sheltered areas. For practical reasons, dry cotton swabs are occasionally used for blood collection and stored at room temperature until arrival in the laboratory. Detection of PRRSV RNA is a function of viral concentration, sample type and storage condition. To evaluate a possible impact of the sampling protocol on PRRSV1 detection, experimentally spiked blood samples using three dilutions of a representative PRRSV1 strain were prepared. In addition, blood samples from pigs naturally infected with PRRSV were obtained from a PRRSV-positive British herd. Spiked blood and blood from infected pigs were used to obtain sera, dry or wet (immersed in saline) polyester or cotton swabs and FTA cards. The different samples were stored for 24 hours, 48 hours or 7 days at 4°C or 20°C and tested by a real-time reverse transcriptase PRRSV PCR assay. Under the study conditions, the best matrix was serum (96.7 per cent), followed by wet swabs (78 per cent), dry swabs (61.3 per cent) and FTA cards (51 per cent). Polyester swabs (76 per cent) showed a better performance than cotton swabs (63.3 per cent). The reduction in sensitivity obtained for swabs and FTA cards was particularly high at low viral concentrations. The results indicate that wet polyester swabs should be used whenever possible.

  • infectious diseases
  • pcr
  • pigs
  • diagnostics
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Footnotes

  • Funding Funding was provided by the Biotechnology and Biological Sciences Research Council (BBSRC) Institute Strategic Programme Grant awarded to the Roslin Institute (BB/J004324/1; BBS/E/D/20241864).

  • Competing interests None declared.

  • Provenance and peer review Not commissioned; externally peer reviewed.

  • Correction notice This article has been corrected since it was published Online First. The title has been updated.

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