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How does insulin increase the risk of laminitis?

C. L. Baskerville, S. Chockalingham, P. A. Harris and others

PeerJ (2018) 6

doi: 10.7717/peerj.5945

• What did the research find?

Insulin-like growth factor 1 (IGF-1) receptors were found to be present on lamellar epithelial cells. Insulin caused a concentration-dependent increase in lamellar epithelial cell proliferation, with the maximum effect observed at an insulin concentration of 1000 μIU/ml. Coincubation with a blocking antibody to the IGF-1 receptor significantly inhibited the proliferative effect of insulin.

• How was it conducted?

Immunohistochemistry was performed on formalin-fixed lamellar tissue sections from the forelimbs of six healthy horses obtained from a local abattoir, using an anti-IGF-1 receptor antibody. Lamellar epithelial cells were obtained from the hooves of a further 18 healthy horses. These cells were then incubated with insulin (0 to 2000 μIU/ml) for 48 hours. Some cells were also incubated with an anti-IGF-1 receptor antibody to prevent insulin binding. Cellular proliferation was …

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