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Bacteria profile and antibiogram of the bacteria isolated from the exposed pulp of dog canine teeth
  1. José Carlos Almansa Ruiz1,
  2. Annelize Jonker2,
  3. Anna-Mari Bosman2 and
  4. Gerhard Steenkamp1
  1. 1 Department of Companion Animal Clinical Studies, Faculty of Veterinary Science, University of Pretoria, Onderstepoort, Pretoria, South Africa
  2. 2 Department of Tropical Diseases, Faculty of Veterinary Science, University of Pretoria, Onderstepoort, Pretoria, South Africa
  1. E-mail for correspondence;{at}


Twenty-seven microbiological samples were taken from root canals (RC) of the canine teeth of 20 dogs where the pulps were non-vital and exposed due to complicated crown fractures. These pulps were cultured for aerobic/anaerobic bacteria. Antimicrobial susceptibility of isolates was determined using the Kirby-Bauer diffusion test. A total of 49 cultivable isolates, belonging to 27 different microbial species and 18 different genera, were recovered from the 27 RCs sampled. Twenty (40.81 per cent) of the cultivable isolates were Gram positive while 29 (59.19 per cent) were Gram negative. Facultative anaerobes were the most common bacteria (77.56 per cent). Aerobic isolates represented 18.36 per cent, and strict anaerobes 4.08 per cent. The antimicrobials with the highest in vitro efficacy were gentamicin (100 per cent) and enrofloxacin (93.32 per cent).

  • dogs
  • root canal
  • bacteria
  • non-vital pulp
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  • Funding The research was funded by The Peter Emily International Veterinary Dental Foundation, Department of Companion Animal Clinical Studies of the University of Pretoria, and the University of Pretoria Research Fund.

  • Competing interests None declared.

  • Ethics approval The study was approved by the Animal Use and Care Committee, and the Research Committee of the University of Pretoria (V061/10).

  • Provenance and peer review Not commissioned; externally peer reviewed.

  • Author note This manuscript represents a portion of a thesis submitted by JCAR to the Department of Companion Animal Clinical Studies, University of Pretoria, as partial fulfilment of the requirements for a Master of Science degree.

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