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SALMONELLA species have been isolated from human, cattle and goat gallbladders (Chandra and others 2006, Dias and others 2013, Gonzalez-Escobedo and Gunn 2013). Salmonella species genetically regulate mechanisms of resistance to the antibacterial and detergent-like properties of bile salts, thus they survive for a long time in this hostile environment (Begley and others 2005). They do it, either by down-regulating secretion and invasion genes in the type III secretion system of pathogenicity (Prouty and Gunn 2000) or by forming protective biofilms on gallstones (Crawford and others 2010). Their survival leads to lengthy intermittent shedding of pathogenic serovars or multidrug resistant strains (Gonzalez-Escobedo and Gunn 2013).
Therefore, the objective of this study was to investigate the presence of Salmonella species serovars in the gallbladder of slaughtered pigs and evaluate their antimicrobial resistance, thus their public health importance.
One hundred and twenty-three gallbladders collected from randomly selected healthy pigs at slaughter over a period of 6 months were cultured following ISO 6579 (ISO 2002) (Annex D). Suspect colonies were subcultured on nutrient agar, confirmed as Gram negative, tested for oxidase production, utilisation of Triple Sugar Iron Agar (Merck, Germany) and assigned to species using the Microgen systems GnA and B-ID (Microgen …
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