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Origin of porcine circovirus type 2 (PCV2) from swine affected by PCV2-associated diseases in Croatia
  1. D. Novosel, PhD, DVM1,
  2. T. Tuboly, PhD, DVM, Prof2,
  3. A. Csagola, PhD, DVM2,
  4. M. Lorincz, DVM2,
  5. V. Cubric-Curik, PhD, Prof3,
  6. A. Jungic, DVM4,
  7. I. Curik, PhD, Prof3,
  8. J. Segalés, PhD, DVM, Dipl. ECPHM, Dipl. ESVP, Prof5,
  9. M. Cortey, PhD, MSc, BSc6 and
  10. Z. Lipej, DVM, PhD1
  1. 1Department of Pathology, Croatian Veterinary Institute, Zagreb 10000, Croatia
  2. 2Department of Microbiology and Infectious Diseases, Faculty of Veterinary Science, Szent István University, Budapest, Hungary
  3. 3Department of Animal Science, Faculty of Agriculture, University of Zagreb, Zagreb, Croatia
  4. 4Virology Department, Croatian Veterinary Institute, Zagreb, Croatia
  5. 5Centre de Recerca en Sanitat Animal (CReSA), UAB-IRTA, Barcelona, Spain
  6. 6Unité de Virologie Moléculaire, Emergence et Co-évolution Virale, Marseille, France
  1. E-mail for correspondence: dinko.novosel{at}


Porcine circovirus type 2 (PCV2) causes some of the most significant economic losses in pig production. Several multisystemic syndromes have been attributed to PCV2 infection, which are known as PCV2-associated diseases (PCVDs). This study investigated the origin and evolution of PCV2 sequences in domestic pigs and wild boars affected by PCVDs in Croatia. Viral sequences were recovered from three wild boars diagnosed with PCV2-systemic disease (PCV2-SD), 63 fetuses positive for PCV2 DNA as determined by PCR, 14 domestic pigs affected with PCV2-SD (displaying severe interstitial nephritis) and five domestic pigs with proliferative and necrotising pneumonia. Seventeen complete PCV2 genomes were recovered. Phylogenetic and evolutionary analyses based on median-joining phylogenetic networks, amino acid alignments and principal coordinate analysis were performed using complete genomes, as well as complete and partial ORF sequences for ORF1 and ORF2. Two of the 17 PCV2 sequences belonged to PCV2a, 14 to PCV2b and one was unclustered. PCV2b was the predominant genotype in Croatia and has been linked to international trade as a route of introduction. Correlation between particular viral strains with PCVDs is lacking.

  • Porcine circovirus type 2
  • Phylogenetic analysis
  • Porcine circovirus-associated disease

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