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One of the authors, Simone Schuller, comments: I would like to thank Professor Prescott for his valid comments on the limitations of the use of dark field microscopy (DFM) for determination of urinary shedding of leptospires with reference to the case recently described by Juvet and others (2011).
We agree that the use of DFM as a diagnostic tool can be problematic because of limited sensitivity and specificity (Faine and others 2000). Besides …
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