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Comparative evaluation of three commercially available complement fixation test antigens for the diagnosis of glanders
  1. I. Khan, DVM, MSc1,
  2. L. H. Wieler, DrMedVet2,
  3. F. Melzer, DrMedVet1,
  4. M. Gwida, DVM, MVSc, DrMedVet1,
  5. V. L. de. A. Santana, MedVet, PhD3,
  6. M. M. A. de Souza, Med Vet3,
  7. M. Saqib, DVM, MSc, PhD4,
  8. M. C. Elschner, DrMedVet1 and
  9. H. Neubauer, DrMedVet1
  1. Friedrich-Loeffler-Institute, Federal Research Institute for Animal Health, Institute of Bacterial Infections and Zoonoses, Naumburger Strasse 96a, 07743 Jena, Germany
  2. Institute of Microbiology and Epizootics, Free University, Berlin, Philippstrasse 13, 10115 Berlin, Germany
  3. Laboratório Nacional Agropecuário Unidade de Diagnóstico Bacteriológico, Ministério da Agricultura, Pecuária e Abastecimento, Rua Dom Manoel De Medeiros, S/N Dois Irmãos, Recife PE CEP 52171-030, Brazil
  4. Department of Clinical Medicine and Surgery, University of Agriculture, Faisalabad 38040, Pakistan
  1. E-mail for correspondence drkhan_uaf{at}

The sensitivity and specificity of three commercially available complement fixation test (CFT) antigens from (, Central Veterinary Institute of Wageningen UR (CIDC) and the United States Department of Agriculture (USDA) were comparatively evaluated by testing 410 sera collected from glanders-endemic and non-endemic areas (200 true-negative randomly collected sera and 210 sera collected from experimentally immunised animals (12 rabbits, 19 horses), clinically positive (135) and culture-positive (44) horses, donkeys and mules). Immunoblotting (IB) was used as the gold standard test. Highest sensitivity was shown for the CIDC antigen (100 per cent) followed by the antigen (99.39 per cent). However, the USDA antigen showed substantially less (p<0.05) sensitivity (62.19 per cent). Highest specificity was found for the USDA antigen (100 per cent) followed by the CIDC (97.5 per cent) and antigen (96.5 per cent). Positive and negative predictive values (assumed glanders prevalence of <0.1 per cent) for each antigen were calculated to be 95.88 and 99.48 (, 97.04 and 100 (CIDC), 100 and 76.33 per cent (USDA), respectively. Almost perfect agreement (0.96) was found between CFT using either or CIDC and IB.

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  • Dr Gwida is also at The Department of Hygiene and Zoonoses, Faculty of Veterinary Medicine, Mansoura University 35516, Mansoura, Egypt

  • Provenance not commissioned; externally peer reviewed

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