Article Text

Evaluation of an elisa to assess the intensity of Fasciola hepatica infection in cattle
  1. M. R. Salimi-Bejestani, NSD, DVM, PhD1,
  2. P. Cripps, BVSc, BSc, MSc, PhD, PGCertTLHE, DipECVPH, MRCVS2 and
  3. D. J. L. Williams, BSc, PhD3
  1. 1 School of Veterinary Medicine, University of Semnan, PO Box 35195-363, Semnan, Iran
  2. 2 Department of Clinical Veterinary Science, Faculty of Veterinary Science, University of Liverpool, Leahurst, Neston, Cheshire CH64 7TE
  3. 3 Veterinary Parasitology, Liverpool School of Tropical Medicine/Faculty of Veterinary Science, University of Liverpool, Pembroke Place, Liverpool L3 5QA
  1. Correspondence to Dr Williams


An elisa with a diagnostic sensitivity of 98 per cent and specificity of 96 per cent was evaluated as a means of assessing the intensity of Fasciola hepatica infection in cattle. A total of 294 blood samples were collected from infected cattle at a local abattoir, and the level of infection in each animal was assessed on the basis of the extent of liver pathology and the presence of flukes; 120 blood samples were also collected from uninfected cattle kept on a farm known to be free of F hepatica. The results indicated that there was a significant correlation (P<0·001) between the elisa values and the intensity of infection. Values between 15 and 28 per cent of a positive control sample indicated a low intensity of infection, values between 28 and 50 per cent indicated a medium intensity of infection and values above 50 per cent indicated a high intensity of infection.

Statistics from

Request Permissions

If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.