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Diagnosis of canine leishmaniasis by a polymerase chain reaction technique
  1. X. Roura, DVM1,
  2. A. Sánchez, PhD2 and
  3. L. Ferrer, DVM, PhD, DipECVD2
  1. 1 Veterinary Teaching Hospital
  2. 2 Facultat de Veterinària, Universitat Autònoma de Barcelona, Bellaterra, 08193, Spain


A polymerase chain reaction (PCR) technique for the diagnosis of canine leishmaniasis on bone marrow samples was developed which amplified a 120 bp DNA fragment of the Leishmania kinetoplast DNA, common to all Leishmania species. Forty-five of 46 dogs in which leishmaniasis had been diagnosed were positive with the PCR technique, whereas none of 41 healthy dogs gave a positive result. Fifteen dogs with leishmaniasis that had been treated for six months with N-methylglucamine antimoniate and allopurinol were also investigated. Seven were positive, implying that they remained infected despite the resolution of their clinical signs.

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