TABLE 4:

Results of real-time RT-PCR and the ICA kit for detection of PEDV in experimentally infected piglets

PigsTestDays postinoculation
0123456789
ASign*n.c.†s.d.s.d.s.d.D
PCR‡+++
Ct§38.422.518.621.3
ICA+++
BSignn.c.s.d.s.d.s.d.s.d.s.d.m.d.m.d.m.d.n.c.
PCR++++++
Ct37.225.422.126.022.721.925.738.639.740.2
ICA++++++
CSignn.c.s.d.s.d.s.d.s.d.D
PCR++++
Ct39.220.916.518.321.8
ICA++++
DSignn.c.s.d.D
PCR+
Ct38.419.3
ICA+
ESignn.c.s.d.s.d.s.d.s.d.s.d.m.d.m.d.D
PCR++++++
Ct41.621.619.416.017.121.723.027.3
ICA++++++
FSignn.c.s.d.s.d.D
PCR++
Ct40.522.615.7
ICA++
GSignn.c.s.d.s.d.s.d.D
PCR+++
Ct39.022.818.417.3
ICA+++
HSignn.c.s.d.s.d.s.d.s.d.s.d.m.d.m.d.m.d.D
PCR++++++
Ct42.2.24.922.118.216.420.023.729.228.4
ICA++++++
  • *Clinical sign.

  • †No clinical signs.

  • ‡Real-time RT-PCR.

  • §Threshold cycle value of real-time RT-PCR.

  • D, death; ICA, immunochromatographic assay; m.d., mild diarrhoea; PEDV, porcine epidemic diarrhoea virus; s.d., severe diarrhoea.