The detection of CODD-associated treponemes following treatment with different chemicals
| Treatment | N | Positive culture, N (%) | Treponeme PCR positive, N (%) | 16S rRNA PCR positive, N (%) |
|---|---|---|---|---|
| Cold water | 10 | 10 (100) | 10 (100) | 10 (100) |
| Hot water | 10 | 10 (100) | 10 (100) | 10 (100) |
| Hand soap | 10 | 0 (0) | 1 (10) | 1 (10) |
| Ethanol | 10 | 0 (0) | 0 (0) | 0 (0) |
| FAM 30 | 10 | 0 (0) | 0 (0) | 0 (0) |
| Virkon | 10 | 0 (0) | 1 (10) | 1 (10) |
Ten swabs were taken from gloves which were known to be positive for treponeme growth following treatment with the chemical. Treponeme growth in culture was determined by specific phylogroup PCR, and the swabs were also exposed to phylogroup-specific PCRs
CODD, contagious ovine digital dermatitis