TABLE 1:

Phase contrast microscopic and PCR analysis of swabs taken from gloves used to handle sheep feet affected by or unaffected by CODD in the case-control experiment

Sample IDAnimal status (CODD+/−)Farm ID numberMicroscopyBacterial culture PCR resultsSwab PCR results
Treponeme genusDD1DD2DD3Treponeme genusDD1DD2DD3Dichelobacter nodosusFusobacterium necrophorum
a+1++++++++++
b+1+++++++++
c+2+++++++++
d2+++
e+2+++++
f2+
g+2+++++++++
h+2+++
i2++
j+2+++++++++
k2++
13++
23+++
33+++
43++
53
73
83+
93++
103+++
113++
123++
133+
143++
163
173
183+
193++
203+
213+++
223+++
243+
253++
263+++
273++
283++
293++
31+3++++++++++
32+3+++++++
33+3++++++++
34+3++++++
35+3+++++++++
36+3++++++
37+3++++++++++
38+3++++++++
39+3++++++++++
40+3+++++++++
10B+2+++++++++
20B+4+++++++++
9B+4++++++
21B+2++++++
29B+4+++++++++
30B+2++++++
  • DD1 refers to the Treponema medium phylogroup, DD2 refers to the Treponema phagedenis phylogroup and DD3 refers to the Treponema pedis phylogroup

  • CODD, contagious ovine digital dermatitis