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Interlaboratory ring trial to evaluate CFT proficiency of European laboratories for diagnosis of glanders in equids
  1. K. Laroucau, PhD1,
  2. C. Colaneri1,
  3. M. Jaÿ, DMV2,
  4. Y. Corde2,
  5. A. Drapeau2,
  6. B. Durand, DMV, PhD3,
  7. S. Zientara, DMV, PhD1 and
  8. C. Beck, DMV1
  9. and European Union laboratories involved in glanders serodiagnosis
  1. 1European Union Reference Laboratory for Equine Diseases, ANSES, Laboratory for Animal Health, Maisons-Alfort, France
  2. 2 Bacterial Zoonosis Unit, Paris-Est University, ANSES, Laboratory for Animal Health, Maisons-Alfort, France
  3. 3Epidemiology Unit, Paris-Est University, ANSES, Laboratory for Animal Health, Maisons-Alfort, France
  1. Correspondence to E-mail for correspondence: karine.laroucau{at}anses.fr

Abstract

To evaluate the routine complement fixation test (CFT) used to detect Burkholderia mallei antibodies in equine sera, an interlaboratory proficiency test was held with 24 European laboratories, including 22 National Reference Laboratories for glanders. The panels sent to participants were composed of sera with or without B mallei antibodies. This study confirmed the reliability of CFT and highlighted its intralaboratory reproducibility. However, the sensitivity of glanders serodiagnosis and laboratory proficiency may be improved by standardising critical reagents, including antigens, and by developing a standard B mallei serum.

  • Burkholderia mallei
  • Complement fixation test
  • Glanders
  • Ring trial
  • Serology
  • Accepted April 7, 2016.

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