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Immunohistochemical localisation and effect of matrix metalloproteinases and their inhibitors on canine spontaneous periodontitis
  1. S. M. Kaiser, Dr. med. vet.1,
  2. C. Thiel, Dr. med. vet., Dipl. ECVS1,
  3. M. Kramer, Prof. Dr. med. vet. Dr. h. c., Dipl. ECVDI1,
  4. B. B. Raddatz, PhD2,
  5. K. Failing, Dr. rer. nat.3 and
  6. S. Alldinger, PD Dr. med. vet., Dipl. ECVP4
  1. 1Department of Veterinary Clinical Science, Small Animal Clinic, Justus-Liebig-University, Frankfurter Straße 108, Giessen 35392, Germany
  2. 2Department of Pathology, University of Veterinary Medicine, Bünteweg 17, Hannover 30559, Germany
  3. 3Department for Biomathematics and Data Processing, Veterinary Division, Justus-Liebig-University, Frankfurter Straße 95, Giessen 35392, Germany
  4. 4German Veterinary Medical Society, Friedrichstraße 17, Giessen 35392, Germany
  1. E-mail for correspondence: Susanne.M.Kaiser{at}


Periodontitis is commonly observed in dogs. In human medicine, it is well documented that matrix metalloproteinases (MMPs) are involved in the destruction of the periodontium. Therefore, the aim of this prospective study was to investigate the impact of MMPs and their inhibitors, the TIMPs (tissue inhibitors of metalloproteinases), on canine periodontitis. The oral cavities of 57 dogs were examined clinically and radiologically. Gingival biopsies were obtained from the examined dogs and histologically analysed via haematoxylin and eosin stained sections. Immunohistological detection of MMP-2, MMP-3, MMP-8 and MMP-9 as well as TIMP-1 and TIMP-2 was performed by the avidin-biotin peroxidase complex technique. All sections were evaluated by light microscopy. Statistically significant positive correlations were detected between the histologically verified degree of inflammation and the expression of MMP-2, MMP-3, MMP-8 and MMP-9 as well as between changes in collagen fibre content and the occurrence of MMP-2, MMP-8 and MMP-9. Concerning TIMP-1 and TIMP-2, non-significant, generally negative correlations were observed. In summary, in canine periodontitis, an increased expression of the above mentioned MMPs and a tendentially decreased expression of TIMPs are present. In conclusion, in canine periodontitis, a MMP-TIMP imbalance is suggestive of contributing to the destruction of the periodontium.

  • matrix metalloproteinases
  • dog
  • periodontitis
  • immunohistology

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