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Border disease virus (BDV), together with bovine viral diarrhoea virus 1 (BVDV-1), BVDV-2 and classical swine fever virus (CSFV), is classified in the genus Pestivirus within the family Flaviviridae. Pestiviruses have been traditionally classified according the species that they infect, but interspecies transmission of pestiviruses among several artiodactyla species has been widely reported. Thus, whereas CSFV is predominantly restricted to pigs, BVDV and BDV infect many different ruminant species as well as swine (Becher and others 1997). CSFV serological surveillance is carried out in Spain according to the regulations issued by the European Union. In 2006, healthy pigs from a fattening swine farm in northern Spain tested positive in a routine ELISA screening for antibodies against CSFV. In a visit to the farm, no clinical signs (with no abnormal pig losses or low productivity) were observed. The positive sera were further analysed using the virus neutralisation test (VNT), and the antibody titres against the viruses used did not show clear-cut differentiation between CSFV and other pestiviruses. To monitor pestivirus infection in the herd, and to characterise the virus, additional serological and virological studies were performed. The present study focused on antigenic and molecular characterisation of pestivirus isolated from naturally infected pigs from a herd in northern Spain detected during the CSFV surveillance programme.
One-month-old piglets were monitored for pestivirus infection on four sampling dates (SD1: 533 pigs, SD2: 523 pigs, SD3: 509 pigs, SD4: 470 pigs) at intervals of 45 days. To detect pestivirus at the …