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Diagnosis of botulism types C and D in cattle by a monoclonal antibody-based sandwich ELISA
  1. C. E. Brooks, BSc,
  2. H. J. Clarke, BA, MSc,
  3. D. A. Graham, MVB, PhD, MRCVS and
  4. H. J. Ball, BSc, MSc, PhD, FRCPath
  1. Veterinary Sciences Division, Agri-Food and Biosciences Institute Northern Ireland, Stoney Road, Stormont, Belfast BT4 3SD
  1. E-mail for correspondence cathy.brooks{at}

This study was undertaken to evaluate two monoclonal antibody-based sandwich ELISAs (sELISAs) for the detection of Clostridium botulinum neurotoxins (BoNTs) types C and D from culture-enriched intestinal content samples from cattle. To validate the diagnostic significance of the presence of cultivable, toxin-producing C botulinum in the intestines of cattle, samples from both suspect and non-suspect botulism cases were examined. BoNT was detected by both sELISAs in a greater number of suspect animals than by direct testing of uncultured samples by mouse bioassay. One sELISA detected two BoNT C and one BoNT Group III mosaic isoform in three animals that were missed by the other, and both sELISAs failed to identify samples from two mouse bioassay-positive BoNT C animals. BoNT D was also detected in one non-suspect sample by one of the sELISAs.

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  • Provenance not commissioned; externally peer reviewed

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