The detailed clinical and epidemiological history and the pathology of neonatal canine herpesvirus (CHV) disease in a naturally infected litter of pups is reported. Herpesvirus was isolated from the brain, lung, liver, spleen, kidney and intestine of one of the affected litter which died on day 10 after whelping. The isolation of virus correlated with the distinctive gross and histological changes in these organs. Herpesvirus was isolated also from the anterior vagina of the bitch 18 days after whelping --a finding which may be of significance in the epidemiology of the natural disease.
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