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Development of rapid immunochromatographic strip test for the detection of porcine epidemic diarrhoea virus
  1. Kwang-Soo Lyoo, DVM, PhD1,
  2. Minjoo Yeom, MSc2,
  3. Jungho Kim, PhD3,
  4. Donghyuk Kim, MSc3,
  5. Gunwoo Ha, PhD3,
  6. Woonsung Na, DVM, PhD2,
  7. Van Phan Le, DVM, PhD4 and
  8. Daesub Song, DVM, PhD2
  1. 1 Korea Zoonosis Research Institute, Chonbuk National University, Jeonju, Jeollabuk-do, Republic of Korea
  2. 2 Department of Pharmacy, College of Pharmacy, Korea University, Sejong, Republic of Korea
  3. 3 BioNote, Hwaseong, Republic of Korea
  4. 4 Department of Microbiology and Infectious Disease, College of Veterinary Medicine, Vietnam National University of Agriculture, Hanoi, Vietnam
  1. E-mail for correspondence; sds1{at}korea.ac.kr

Abstract

Porcine epidemic diarrhoea virus (PEDV) causes acute and severe watery diarrhoea and dehydration, as well as 50–100 per cent mortality in piglets. For the PEDV diagnosis, a rapid test kit that is specific and sensitive to PEDV is critical to monitor this disease at pig farms. The present study aimed to develop an immunochromatographic assay (ICA) strip test for detecting PEDV in faecal swabs. The newly developed diagnostic test showed a detection limit of 104.0 TCID50/ml of PEDV. Using faecal swab samples, the relative sensitivity and specificity of the ICA kit were 95.0 per cent and 98.6 per cent, respectively, compared with those of real-time RT-PCR. In samples from piglets experimentally infected with PEDV, the results showed 100 per cent agreement with those found by real-time RT-PCR. Our developed test strip will be useful for rapid diagnosis and can be used for epidemiological surveillance of PEDV infection.

  • porcine epidemic diarrhea virus
  • immunochromatographic test kit
  • rapid diagnosis

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Footnotes

  • Funding This work was supported by Korea Ministry of Environment (MOE) as Public Technology Program based on Environmental Policy (No E416-00021-0602-0) and Vietnam National Foundation for Science and Technology Development (NAFOSTED) under grant number 106-NN.04-2014.16.

  • Competing interests None declared.

  • Provenance and peer review Not commissioned; externally peer reviewed.

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