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Antimicrobial susceptibility of Mycoplasma bovis isolates from cattle in the Netherlands
A. Heuvelink, C. Reugebrink, J. Mars
IN cattle, Mycoplasma bovis is able to cause respiratory disease, mastitis, arthritis, otitis and reproductive disorders, and is frequently implicated in cases of bovine respiratory disease (BRD) in calves. There is no effective vaccine; therefore, control of M bovis infections depends on good husbandry practices and antibiotic treatment.
To ensure the prudent use of antimicrobial agents, it is important to effectively target antimicrobial treatment. However, assessing the antimicrobial susceptibility of mycoplasmas is difficult due to their slow growth, small size and complex growth media requirements. Given that there are no recent data on susceptibility patterns of M bovis isolated from clinical samples in the Netherlands, the objective of this study was to examine the antimicrobial susceptibility of M bovis field isolates, with a focus on antimicrobial agents approved to treat BRD in the Netherlands.
Ninety-five M bovis isolates were tested that originated from the lungs of animals with respiratory problems (n=56), from mastitic milk (n=27), and from synovial fluid from animals with swollen joints (n=12). Minimal inhibitory concentrations (MICs) were assessed by broth microdilution, using both plates prepared in-house and commercially available MIC plates. For each …