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MENINGIOMAS are a common CNS neoplasm in both humans and dogs. The propagation of meningiomas has been linked with several inflammatory interleukins; human meningioma cells have been identified to synthesise and secrete interleukin-6 (IL-6) (Boyle-Walsh and others 1994, 1996, Todo and others 1994), a proinflammatory cytokine with pleiotropic function, as well as express interleukin-8 (IL-8 or CXCL8) mRNA (Boyle-Walsh and others 1994), an inflammatory chemokine known to enhance angiogenesis by vascular endothelial cell proliferation and tumour neovascularisation (Xie 2001, Brat and others 2005). Although IL-6 and IL-8 expression has been reported in human meningiomas, no comparative information, to the authors’ knowledge, is available currently for canine meningiomas.
The aims of this project were to determine whether canine intracranial meningiomas express IL-6 and or IL-8 and, if so, whether the expression varies among meningioma tumour types and grades in a quantifiable manner.
Nineteen surgically excised formalin-fixed, paraffin-embedded canine intracranial meningioma biopsy samples were collected from the Department of Pathology, the University of Georgia, College of Veterinary Medicine archives (2005–2011). The diagnosis of each tumour was confirmed and classified morphologically by the World Health Organization (WHO) classification scheme for meningiomas (Louis and others 2007) by a board-certified veterinary pathologist (Howerth).
Immunohistochemical detection of IL-6 and IL-8 was performed by an automated staining system (DakoCytomation, Carpinteria, California, USA) using anti-canine IL-6 (1:2000, monoclonal mouse, R&D Systems, Minneapolis, Minnesota, USA) and anti-canine IL-8 (1:40, monoclonal mouse, R&D Systems, Minneapolis, Minnesota, USA) antibodies, respectively. The negative controls were the tissue …