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Serotypes and antimicrobial resistance patterns of the recent Korean Actinobacillus pleuropneumoniae isolates
  1. A. N. Yoo, DVM, MS1,
  2. S. B. Cha, DVM, PhD1,
  3. M. K. Shin, DVM, PhD1,
  4. H. K. Won, DVM, MS, PhD1,2,
  5. E. H. Kim, PhD2,
  6. H. W. Choi, DVM, MS, PhD2 and
  7. H. S. Yoo, DVM, MS, PhD1
  1. 1Department of Infectious Diseases, College of Veterinary Medicine and Brain Korea 21 Program for Veterinary Science, Seoul National University, Seoul 151-742, Korea
  2. 2ChoongAng Vaccine Laboratory Co. Ltd., Daejeon 305-348, Korea;
  1. E-mail for correspondence: yoohs{at}snu.ac.kr

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In Korea, the addition of antibiotics to feed was banned in July 2011; since then, reports of outbreaks of porcine pleuropneumonia have increased. Therefore, knowledge of the characteristics of clinical isolates emerging in recent years is needed. This study analysed the serotype and antimicrobial susceptibility of 102 Actinobacillus pleuropneumoniae isolates from pigs with pleuropneumonia in Korea from 2006 to 2010. Serotype 5 was the most frequently isolated but the incidence of serotype 1 significantly increased in 2010. The susceptibility to florfenicol decreased considerably from 2006 to 2010, as did that of a minor percentage of isolates to ampicillin and amoxicillin. Of 11 tetracycline resistance genes, tet (B) was predominant (62 per cent), followed by tet (H) and tet (O). The florfenicol resistance (flo R) gene (34 per cent) and β-lactam antibiotic resistance (blaROB-1) gene (15 per cent) were also detected. The expression levels of the tetracycline repressor gene and resistance gene were not significantly correlated with the minimum inhibitory concentration values of tetracycline-resistant strains. Interspecies transferability of the resistance genes (flo R, tet (B)/blaROB-1) was observed with frequencies of 5.7×10−3 and 3.5×10−2, respectively. In analysing DNA patterns using pulsed field gel electrophoresis, there was a slight positive correlation with serotypes but not with isolated location and year. This study acknowledges the emergence of new serotype and antimicrobial resistance of A pleuropneumoniae based on the current situation in Korea. This knowledge may be useful in controlling the disease caused by A pleuropneumoniae.

Introduction

Actinobacillus pleuropneumoniae is a causative agent of porcine pleuropneumonia, a respiratory disease in pigs of all ages that may result in sudden death often detected in the postmortem inspection at slaughter, or in severe symptoms characterised by fibrinous pleuritis and haemorrhagic and fibrinous pneumonia with necrotic lung lesions (Bosse and others 2002 …

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