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Using acetate tape to collect samples for the diagnosis of demodicosis in dogs

A. V. Pereira, S. A. Pereira, I. D. F. Gremião, M. P. Campos, A. M. R. Ferreira

THE diagnosis of canine demodicosis is commonly made by visualising the demodectic mite and its developmental stages in deep skin scrapings. It has been reported that squeezing the skin can increase the number of mite-positive samples in dogs with demodicosis. Acetate tape impression is another technique that has been used for the detection of extrafollicular mites. The aim of this study was to compare the sensitivities of acetate impression with skin squeezing and deep skin scraping with skin squeezing for the diagnosis of canine demodicosis.

A group of 30 dogs with clinical signs of demodicosis were included in the study. A lesion on the head, trunk or forelimb was selected for sampling for both techniques. For the acetate tape impression, a 10 cm piece of clear, pressure-sensitive tape was placed on the selected lesion and the skin was then squeezed. The tape strip was removed, fixed on a slide and examined under a microscope. Deep skin scraping was performed by firmly squeezing the previously selected lesion (adjacent to the site used for tape impression) to …

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