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Zoonotic Diseases
PCR detection of leptospiral carriers among seronegative horses
  1. C. Hamond1,
  2. G. Martins1,
  3. W. Lilenbaum1 and
  4. M. A. Medeiros2
  1. 1Laboratory of Veterinary Bacteriology, Department of Microbiology and Parasitology, Universidade Federal Fluminense, 24210-130, Brazil
  2. 2Bio-Manguinhos, Oswaldo Cruz Foundation, Brazilian Ministry of Health, Rio de Janeiro, RJ 21040-360, Brazil
  1. e-mail: mipwalt{at}vm.uff.br

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WE recently published a short communication about bovine leptospirosis demonstrating that, although serology was an acceptable screening tool for leptospirosis on a herd basis, it was not a good predictor for detecting bovine carriers at an individual level, since 50 per cent of the cows that were shedding leptospires in urine, using PCR, were seronegative (Otaka and others 2012).

Considering these data, we have decided to compare serology with PCR in a broader study conducted in horses. The clinical syndrome of leptospirosis …

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