Article Text

PDF
Paper
Detection of elephant endotheliotropic herpesvirus type 1 in asymptomatic elephants using TaqMan real-time PCR
  1. K. Hardman, BVetMed, MSc, MRCVS1,
  2. A. Dastjerdi, DVM, MSc, PhD2,
  3. R. Gurrala, BSc, MSc, PhD3,
  4. A. Routh, BVSC, CertZooMed, MRCVS4,
  5. M. Banks, PhD5,
  6. F. Steinbach, PhD, MRCVS6 and
  7. T. Bouts, DVM, MSc, DiplECZM, MRCVS7
  1. Institute of Zoology, Zoological Society of London, Regents Park, London, NW1 4RY, UK
  2. Virology Department, Animal Health and Veterinary Laboratories Agency (AHVLA) – Weybridge, New Haw, Addlestone, Surrey, KT15 3NB, UK
  3. Division of Microbial Sciences, Faculty of Health and Medical Sciences, University of Surrey, Guildford, Surrey, GU2 7XH, UK
  4. Veterinary Department, Zoological Society of London, ZSL London Zoo, London, NW1 4RY, UK
  5. Virology Department, AHVLA – Weybridge, New Haw, Addlestone, Surrey, KT15 3NB, UK
  6. Virology Department, AHVLA – Weybridge, New Haw, Addlestone, Surrey, KT15 3NB, UK
  7. Veterinary Department, Zoological Society of London, ZSL Whipsnade Zoo, Dunstable, Bedfordshire, LU6 2LF, UK
  1. Correspondence to Bouts, e-mail: tim.bouts{at}zsl.org

This study assessed the feasibility of identifying asymptomatic viral shedders using a novel TaqMan real-time PCR on trunk washes and swabs from the conjunctiva, palate and vulva of elephants. Six elephants from a UK collection were sampled weekly over a period of 11 weeks for this study. The herd prevalence of elephant endotheliotropic herpesvirus-1 (EEHV-1) was 100 per cent by PCR. The virus DNA was detected in all the sampling sites; however, the prevalence of virus DNA in the conjunctiva swabs was higher. In addition, Asian elephants from two continental European collections were sampled once and one animal tested positive on a trunk wash. The virus from this animal was phylogenetically typed as EEHV-1A based on 231 nucleotides of the terminase gene.

  • Accepted September 21, 2011.

Statistics from Altmetric.com

  • Accepted September 21, 2011.
View Full Text

Footnotes

  • Ms Hardman is also at The Royal Veterinary College, Royal College Street, London, NW1 0TU, UK

  • Dr Gurrala is also at The Virology Department, AHVLA – Weybridge, New Haw, Addlestone, Surrey, KT15 3NB, UK

  • Provenance not commissioned; externally peer reviewed

Request permissions

If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.