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Effects of different antimicrobial treatments on serum acute phase responses and leucocyte counts in pigs after a primary and a secondary challenge infection with Actinobacillus pleuropneumoniae
  1. M. Sjölund, DVM, PhD1,
  2. C. Fossum, PhD2,
  3. A. J. Martín de la Fuente, DVM, PhD3,
  4. M. Alava, PhD4,
  5. H. R. Juul-Madsen, PhD5,
  6. F. Lampreave, PhD4 and
  7. P. Wallgren, DVM, PhD1
  1. Department of Animal Health and Antimicrobial Strategies, National Veterinary Institute, 751 89 Uppsala, Sweden
  2. Section of Immunology, Department of Biomedicine and Veterinary Public Health, Swedish University of Agricultural Sciences, PO Box 588, Biomedical Center, 751 23 Uppsala, Sweden
  3. Unidad de Microbiología e Inmunología, Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad de León, 24007 León, Spain
  4. Departamento de Bioquimica, Facultad de Ciencias, Universidad de Zaragoza, Pedro Cerbuna 12, 50009 Zaragoza, Spain
  5. Department of Animal Health and Bioscience, Faculty of Agricultural Sciences, Aarhus University, Blichers Allé 20, Postboks 50, 8830 Tjele, Denmark
  1. E-mail for correspondence marie.sjolund{at}
  • Dr Sjölund and Professor Wallgren are also at the Department of Clinical Sciences, Swedish University of Agricultural Sciences, PO Box 7054, 750 07 Uppsala, Sweden

The susceptibility to an initial challenge and a re-challenge inoculation with Actinobacillus pleuropneumoniae was analysed in pigs that were treated with antimicrobials of different efficacies following the first exposure to A pleuropneumoniae. In brief, 30 nine-week-old specific pathogen-free pigs were allocated to five groups of six. After acclimatisation, four groups were inoculated with A pleuropneumoniae serotype 2. At the onset of clinical signs, three of the groups of pigs were treated with enrofloxacin, tetracycline or penicillin. A fourth group served as the inoculated control and the fifth group as a control group that had not been inoculated. On day 28, all five groups were re-challenged with the same strain of A pleuropneumoniae serotype 2 as had been used in the first inoculation. No treatments were carried out at this time. The acute phase responses and differential leucocyte counts were monitored in detail after both inoculations. Leucocytosis and acute phase responses in the forms of serum amyloid A, pig-major acute phase protein and haptoglobin were recorded in all of the inoculated groups after the onset of clinical signs following the first inoculation. A porcine mannan-binding lectin-A response was less evident in the pigs. Acute phase responses resembling those of the first inoculation were observed in the pigs that had not previously been inoculated and in the pigs treated with enrofloxacin. Acute phase responses were not recorded in the other three groups, where the pigs had seroconverted to A pleuropneumoniae serotype 2 following the first inoculation.

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