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Refining dipstick tests in cats to reduce false-positive results
M. Miyazaki, K. Fujiwara, Y. Suzuta, N. Wakamatsu, H. Taira, A. Suzuki, T. Yamashita
URINE dipstick testing is a simple and sensitive method of screening for proteinuria as an indicator of renal disease. In normal cats, however, the kidneys secrete cauxin (a 70 kDa glycoprotein), which is responsible for false-positive protein scores on urine dipstick testing. To overcome this problem, this study developed a Lens culinaris (lentil) agglutinin (LCA) lectin tip to remove cauxin from urine samples from cats.
An affinity gel was developed using LCA, determined to have a high affinity and specificity for cauxin. Both LCA and control tips were produced. The LCA lectin tip was tested using urine samples from 13 clinically normal intact male and female cats. Urine samples from normal intact male cats were supplemented with feline serum to assess whether the lectin tip would remove cauxin but not serum proteins, which are the major component of proteinuria in cases of chronic kidney disease (CKD). Finally, the lectin tip was tested using urine samples from 20 cats with CKD, for which protein scores were +1 or +2 on dipstick analysis. To analyse the protein content of the …
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