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Comparison of tuberculin activity using the interferon-γ assay for the diagnosis of bovine tuberculosis
  1. I. Schiller, DVM1,
  2. H. M. Vordermeier, PhD2,
  3. W. R. Waters, DVM, PhD3,
  4. A. Kyburz, MSc1,
  5. M. Cagiola, DVM4,
  6. A. Whelan, PhD2,
  7. M. V. Palmer, DVM, PhD3,
  8. T. C. Thacker, MS, PhD3,
  9. J. Meijlis5,
  10. C. Carter, DVM6,
  11. S. Gordon, PhD7,
  12. T. Egnuni, DVM2,
  13. R. Hardegger1,
  14. B. Marg-Haufe1,
  15. A. Raeber, PhD,1 and
  16. B. Oesch, PhD1
  1. 1 Prionics, Wagistrasse 27a, 8952 Schlieren-Zurich, Switzerland
  2. 2 Veterinary Laboratories Agency - Weybridge, Woodham Lane, New Haw, Addlestone, Surrey KT15 3NB
  3. 3 National Animal Disease Center, PO Box 70, 2300 Dayton Road, Ames, IA 50010, USA
  4. 4 Istituto Zooprofilattico dell'Umbria e delle Marche, Via G. Salvemini 1, 06126 Perugia, Italy
  5. 5 Lelystad Biologicals, PO Box 405, 8200 AK Lelystad, The Netherlands
  6. 6 AsureQuality, Private Bag 14 946, Panmure, Auckland, New Zealand
  7. 7 College of Life Sciences and Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Dublin, Ireland
  1. Correspondence to Dr Oesch, e-mail:bruno.oesch{at}prionics.com

Abstract

In this study, interferon-γ (IFN-γ) responses in whole blood cultures stimulated with tuberculins from different sources were compared with regard to their diagnostic reliability in cattle experimentally and naturally infected with Mycobacterium bovis. The IFN-γ responses to different concentrations of purified protein derivatives (PPDs) from M bovis and Mycobacterium avium were quantified. Significant differences (P<0.05) between sources and concentrations of PPDs used for stimulation were detected, indicating a need for standardisation of PPDs used in the IFN-γ assay. Additionally, a tool named ‘relative potency 30‘ that allows rapid comparison of batches and sources of PPDs was defined.

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