The use of liquid carbon dioxide (CO2) was evaluated as a means of culling a flock of five-week-old pullets in situ. It took five minutes and 20 seconds for sufficient liquid CO2 to be injected (3.24 tonnes) to achieve the target concentration of 45 per cent CO2. Although very low ambient temperatures were recorded (below -80°C) during gassing, on the basis of postmortem reports and other data it is inferred that the birds died within minutes of exposure to the gas and before experiencing the extremely low temperatures recorded in the house.
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