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Detection by real-time RT-PCR of a bovine respiratory syncytial virus vaccine in calves vaccinated intranasally
  1. C. Belloc, DVM, PhD, DipECPHM,
  2. R Guattéo, DVM, PhD,
  3. N. Bareille, DVM, PhD, DipECBHM,
  4. H. Seegers, DVM, PhD, DipECBHM,
  5. S. Assié, DVM, PhD, DipECBHM1,
  6. A. Douart, DVM, DipECBHM2,
  7. E. Le Dréan, DVM, PhD3,
  8. C. Maingourd, PhD4 and
  9. E. Sellal, DVM5
  1. 1 UMR Bio-aggression, Epidemiology and Risk Analysis
  2. 2 Department of Farm Animal Health and Public Health, Ecole Nationale Vétérinaire de Nantes, BP 40706, 44307 Nantes Cedex 03, France
  3. 3 Laboratoire Départemental D'Analyses d'Ille et Vilaine, 24 rue Antoine Joly, 35000 Rennes, France
  4. 4 Laboratoire Départemental d'Analyses Deux Sàvres, 23 rue Abreuvoir, 79000 Niort, France
  5. 5 Laboratoire Service International, Le Bois Dieu, 69380 Lissieu, France

Abstract

Seventeen four- to five-week-old calves that were not shedding bovine respiratory syncytial virus (BRSV) were vaccinated intranasally against the disease and sampled by nasal swabbing on 16 different days for up to 20 days after vaccination. BRSV vaccine virus was detected in 15 of the 17 calves. Five of the calves were PCR positive on only one swab, eight were PCR positive on two to five swabs and two were PCR positive on more than five swabs. Twelve of the calves were positive only before day 14 and three were positive after day 14. The nasal shedding of BRSV vaccine virus was very variable.

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