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Screening of bovine coagulase-negative staphylococci from milk for superantigen-encoding genes
  1. M. Nemati, DVM1,
  2. K. Hermans, DVM, PhD, MSc1,
  3. D. Vancraeynest, DVM, PhD1,
  4. S. De Vliegher, DVM, PhD, MSc, DiplECVPH2,
  5. O. C. Sampimon, MSc3,
  6. M. Baele, PhD1,
  7. E. M. De Graef, DVM1,
  8. F. Pasmans, DVM, PhD, MSc1 and
  9. F. Haesebrouck, DVM, PhD, DiplECPHM1
  1. 1 Department of Pathology, Bacteriology and Poultry Diseases
  2. 2 Department of Reproduction, Obstetrics and Herd Health, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium
  3. 3 GD Animal Health Service Deventer, Arnsbergstraat 7, PO Box 9, 7400 AA Deventer, The Netherlands
  1. Dr Nemati is also at Department of Bacteriology, Faculty of Veterinary Medicine, Ilam University, Ilam, Iran


A collection of 102 coagulase-negative staphylococci (cns), isolated from cases of subclinical and clinical bovine mastitis and belonging to 10 different species, were screened by pcr for the presence of genes encoding enterotoxins and enterotoxin-like toxins (sea, seb, sec, sed, see, seg, seh, sei, sej, selk, sell, selm, seln, selo, selp, selq and selu), toxic shock syndrome toxin-1 (tst), and exfoliative toxins A and B (eta and etb). No toxin gene sequences were amplified from any of the isolates, indicating that superantigens encoded by genes detectable by the pcr tests used were not involved in the development of subclinical and clinical mastitis in cattle infected with the cns isolates tested.

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