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Molecular diagnosis of Salmonella species in captive psittacine birds
  1. M. C. Allgayer, BVetMed, MS1,
  2. C. A. V. Lima-Rosa, BVetMed, PhD2,
  3. T. A. Weimer, BPharm, PhD1,
  4. C. R. Rodenbusch, BVetMed, MS3,
  5. R. A. Pereira, BVetMed, PhD3,
  6. A. F. Streck, BVetMed3,
  7. S. D. Oliveira, BVetMed, PhD4 and
  8. C. W. Canal, BVetMed, PhD3
  1. 1 Universidade Luterana do Brasil, Avenida Farroupilha 8001, Canoas, CEP 92425-900, RS, Brazil
  2. 2 Universidade do Estado de Santa Catarina, Lages, SC, Brazil
  3. 3 Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil
  4. 4 Pontificia Universidade Católica, Porto Alegre, RS, Brazil

Abstract

Cloacal swabs were collected from 280 captive psittacine birds belonging to 13 species. Samples of dna were tested by pcr using a pair of primers that amplify a 284 base pair fragment of the Salmonella genus invA gene, and the pcr-positive samples were tested by standard microbiological techniques. Thirteen per cent of the samples were positive by pcr, but negative by microbiological techniques. The infection rates were significantly different among the 13 species, the most commonly infected being Amazona amazonica (28 per cent) and Amazona pretrei (20 per cent). Specific tests for Salmonella Typhimurium Salmonella Enteritidis, Salmonella Pullorum and Salmonella Gallinarum did not produce positive results.

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