Sensitive test for screening for Staphylococcus aureus in bovine mastitis by broth cultivation and PCR
- N. Yamagishi, DVM, PhD1,
- Y. Jinkawa, DVM1,
- K. Omoe, DVM, PhD3,
- S. Makino, DVM, PhD2 and
- K. Oboshi, DVM, PhD1
- 1 Research Unit for Food Animal Medicine, Obihiro, Hokkaido 080-8555, Japan
- 2 Research Unit for Foodborne Diseases, Research Centre for Animal Hygiene and Food Safety, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan
- 3 Research Unit for Veterinary Microbiology and Food Safety, Department of Veterinary Medicine, Faculty of Agriculture, Iwate University, Morioka, Iwate 020-8550, Japan
- Dr Yamagishi's present address is Research Unit for Diagnosis and Treatment of Food Animal Diseases, Department of Veterinary Medicine, Faculty of Agriculture, Iwate University, Morioka, Iwate 020-8550, Japan
- Dr Jinkawa's present address is Hokubu Livestock Hygiene Service Centre, Ishikawa Prefecture, 1-47 Otsu Nanao, Ishikawa 929-2126, Japan
Abstract
An assay was developed and evaluated for screening for Staphylococcus aureus in milk samples from cases of bovine mastitis by overnight cultivation in a broth containing 7·5 per cent sodium chloride, followed by pcr to amplify the nuc gene. The assay could detect concentrations of S aureus as low as 1 colony-forming unit/ml milk. Among 106 milk samples collected from individual quarters of lactating cows in one dairy herd and from a bulk tank, S aureus was detected in nine samples by the pcr assay but in only three samples by conventional microbiological culture.
- British Veterinary Association. All rights reserved.
