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Sensitive test for screening for Staphylococcus aureus in bovine mastitis by broth cultivation and PCR
  1. N. Yamagishi, DVM, PhD1,
  2. Y. Jinkawa, DVM1,
  3. K. Omoe, DVM, PhD3,
  4. S. Makino, DVM, PhD2 and
  5. K. Oboshi, DVM, PhD1
  1. 1 Research Unit for Food Animal Medicine, Obihiro, Hokkaido 080-8555, Japan
  2. 2 Research Unit for Foodborne Diseases, Research Centre for Animal Hygiene and Food Safety, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan
  3. 3 Research Unit for Veterinary Microbiology and Food Safety, Department of Veterinary Medicine, Faculty of Agriculture, Iwate University, Morioka, Iwate 020-8550, Japan
  1. Dr Yamagishi's present address is Research Unit for Diagnosis and Treatment of Food Animal Diseases, Department of Veterinary Medicine, Faculty of Agriculture, Iwate University, Morioka, Iwate 020-8550, Japan
  2. Dr Jinkawa's present address is Hokubu Livestock Hygiene Service Centre, Ishikawa Prefecture, 1-47 Otsu Nanao, Ishikawa 929-2126, Japan

Abstract

An assay was developed and evaluated for screening for Staphylococcus aureus in milk samples from cases of bovine mastitis by overnight cultivation in a broth containing 7·5 per cent sodium chloride, followed by pcr to amplify the nuc gene. The assay could detect concentrations of S aureus as low as 1 colony-forming unit/ml milk. Among 106 milk samples collected from individual quarters of lactating cows in one dairy herd and from a bulk tank, S aureus was detected in nine samples by the pcr assay but in only three samples by conventional microbiological culture.

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