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Evaluation of an ELISA to detect antibodies to maedi-visna virus in individual and pooled samples of milk from sheep
  1. M. Mazzei, DVM, PhD1,
  2. M. L. Carrozza, BSc2,
  3. P. Bandecchi, DVM1,
  4. G. Mazzanti, DVM3,
  5. A. Mannelli4 and
  6. F. Tolari, DVM1
  1. 1Dipartimento di Patologia Animale, Profilassi ed Igiene degli Alimente, Università di Pisa,Via delle Piagge 2, 56124 Pisa, Italy
  2. 2Scuola Normale Superiore, Piazza Cavalieri 7, 56124 Pisa, Italy
  3. 3Azienda USL 2,Via per Sant’Alessio, 5510,Monte San Quirico, Lucca, Italy
  4. 4Dipartimento di Produzione Animali, Epidemiologia ed Ecologia, Università di Torino,Via Leonardo da Vinci 44, 10095 Grugliasco (TO), Italy

Abstract

An ELISA was used to detect antibodies to maedi-visna virus in samples of serum and milk from individual sheep; the results obtained indicated that the ELISA can be used to detect antibodies in milk. The assay was also applied to samples of bulk-tank milk; a standard curve was created and used to calculate the seroprevalence of maedi-visna in 11 flocks of sheep and the results were compared with the results obtained by applying the ELISA to individual serum samples. There was good agreement between the seroprevalences calculated from the standard curve for bulk-tank milk and from the individual serum samples.

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