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Effects of hypervaccination with bovine herpesvirus type 1 gE-deleted marker vaccines on the serological response and virological status of calves challenged with wild-type virus
  1. J-F. Toussaint, MSc,
  2. C. Letellier, PhD,
  3. P. Kerkhofs, DVM, PhD1,
  4. H-J. Rziha, PhD and
  5. B. Bauer2
  1. 1 Department of Virology, Veterinary and Agrochemical Research Centre, Groeselenberg 90, B-1180 Brussels, Belgium
  2. 2 Federal Research Centre for Virus Diseases of Animals, Institute of Immunology, Paul-Ehrlich Strasse 28, D-72076 Tübingen, Germany

Abstract

Twenty-four calves were immunised four times with gE-deleted infectious bovine rhinotracheitis marker vaccines before being challenged with small doses of wild-type bovine herpesvirus type 1 (BHV-1). The repeated vaccinations induced strong immunity that prevented detectable virus replication and gE-seroconversion after the challenge infection in most of the calves. The hypervaccinated calves that shed virus after the challenge infection showed no delay in gE-seroconversion compared with unvaccinated control calves. Using a sensitive nested PCR, BHV-1 gE sequences could be detected in the trigeminal ganglia of several of the gE-seronegative, challenge-infected calves, possibly indicating the presence of wild-type BHV-1 DNA.

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