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Prevalence of enteropathic Escherichia coli in dogs with acute and chronic diarrhoea
  1. A. A. Sancak, DVM, PhD1,
  2. H. C. Rutgers, DVM, MS, MRCVS1,
  3. C. A. Hart, MB, PhD, FRCPath2 and
  4. R. M. Batt, BVSc, PhD, FRCVS1
  1. 1 Department of Small Animal Medicine and Surgery, Royal Veterinary College, Hawkshead Lane, North Mymms, Hertfordshire AL9 7TA
  2. 2 Department of Medical Microbiology, University of Liverpool, Duncan Building, Daulby Street, Liverpool L69 3GA
  1. University of Ankara, Ankara, Turkey
  2. Masterfoods, Mars, Waltham-on-the-Wolds, Melton Mowbray, Leicestershire LE14 4RS

Abstract

Samples of faeces from 57 dogs with acute diarrhoea, 82 dogs with chronic diarrhoea, 34 clinically healthy household dogs and 88 kennelled control dogs were analysed by hybridisation, using DNA probes to detect enteropathogenic Escherichia coli (EPEc) and enterotoxigenic E coli (ETEC), verocytotoxin-producing E coli (VTEC), enterohaemorrhagic E coli (EHEC), enteroinvasive E coil (EIEc) and enteroaggregative E coli (EAggEC). Samples of duodenal juice from 60 of the 82 dogs with chronic diarrhoea were also examined. Significantly more of the dogs with diarrhoea were excreting EPEC (acute 35.1 per cent, chronic 31.7 per cent) and VTEC (acute 24.6 per cent, chronic 28 per cent) than the kennelled dogs (EPEC 17.1 per cent, VTEC 0 per cent) or the household control dogs (EPEC 6 per cent, VTEC 5.9 per cent). Enteropathic E coli was also detected in the duodenal juice of 23 of 60 (38.3 per cent) of the dogs with chronic diarrhoea. The EPEC attaching and effacing A (eaeA) gene and the verocytotoxin 1 (Vri) gene coding for vTEC were often found together. There was good agreement between in vitro studies and hybridisation for the detection of eaeA and VTri. Isolates from the dogs with diarrhoea adhered significantly more to Hep-2 cells, and vr -positive strains from the dogs with diarrhoea consistently killed more than 50 per cent of Vero cells.

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