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Isolation of a glycoprotein E-deleted bovine herpesvirus type 1 strain in the field
  1. M. Dispas, DVM1,
  2. F. Schynts, DVM, PhD2,
  3. M. Lemaire, DVM, PhD3,
  4. C. Letellier, PhD4,
  5. E. Vanopdenbosch, DVM1,
  6. E. Thiry, DVM, PhD2 and
  7. P. Kerkhofs, DVM, PhD4
  1. 1 Department Biocontrol/Section Epidemiology
  2. 2 Department of Infectious and Parasitic Diseases, Virology and Epidemiology, FMV, University of Liège, Boulevard de Colonster B43 Bis, 4000 Sart Tilman - Liège, Belgium
  3. 3 UMR960 Departement de Microbiologie Moleculaire, Ecole Nationale Veterinaire, 23, chemin des Capelles, F-31076 Toulouse Cedex 3, France
  4. 4 Department of Virology, Veterinary and Agrochemical Research Centre, Groeselenberg 99, 1180 Brussels, Belgium


During a field trial to evaluate the efficacy of repeated vaccinations with bovine herpesvirus type 1 (BHV-1) marker vaccines, a glycoprotein E (gE)-negative BHV-1 strain was isolated from the nasal secretions of two cows, eight months after vaccination with a gE-negative live-attenuated vaccine, initially given intranasally, then intramuscularly. The strain isolated was characterised using immunofluorescence, restriction analysis and CR. All the techniques used identified the isolated virus as a gE-negative BHV-1 phenotypically and genotypically identical to the Za strain used as a control.

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