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Diagnosis of canine parvovirus by rapid immunomigration on a membrane
  1. A. Lacheretz1,
  2. C. Laperrousaz1,
  3. A. Kodjo1,
  4. N. Brajon2,
  5. D. Crevat2 and
  6. S. Guillossou2
  1. 1 Infectious Pathology Unit, Ecole Nationale Veterinaire de Lyon, 1 Avenue Bourgelat, BP 83, 69280 Marcy l'Etoile, France
  2. 2 Synbiotics Europe, 2 Rue Alexander Fleming, 69367 Lyon cedex 07, France

Abstract

Rapid immunomigration on a membrane was applied to the diagnosis of canine parvovirus (CPV) in 128 samples of faeces containing four strains of parvovirus (two CPV-2a strains, itncluding one vaccine strain, and two CPV-2b strains). The results were compared with the results of haemagglutination and ELISA sandwich techniques. The new test was quick and easy to use, and made it possible to identify both the CPV-2a and CPV-2b strains. Its detection thresholds per gram of faeces corresponded to specific haemagglutination titres of between 320 and 640 and a virus titre of between 104 and 105 CCID 50 (dose required to infect 50 per cent of cell cultures).

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