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Evaluation of a dot ELISA kit for measuring immunoglobulin M antibodies to canine parvovirus and distemper virus
  1. T. Waner, BVSc, PhD, DiplECLAM1,
  2. S. Mazar, MSc2,
  3. E. Nachmias, DVM, BSc3,
  4. E. Keren-Kornblatt, DVM2 and
  5. S. Harrus, DVM, PhD4
  1. 1 Israel Institute for Biological Research, PO Box 19, Ness Ziona 70400, Israel
  2. 2 Biogal Galed Laboratories, Kibbutz Galed 19240, Israel
  3. 3 Rupin Veterinary Hospital, Hadar Am 42943, Israel
  4. 4 Koret School of Veterinary Medicine, Hebrew University of Jerusulem, PO Box 12, Rehovot 76100, Israel

Abstract

A dot ELISA for the detection of immunoglobulin M (IgM) antibodies to canine distemper virus (CDV) and canine parvovirus (cpv) was assessed. The titres of IgM antibodies to CDV and cPv in 100 dogs were measured by the Immunocomb ELISA kit and compared with the results derived from the immunofluorescence assay (IFA). There was a strong correlation between the results of the dot ELISA technique and the IFA (P<0.001). The dot ELISA kit was also used to assess the changes in the levels of immunoglobulin G (IgG) and IgM antibodies to cpv and CDv in 10 puppies vaccinated with a polyvalent vaccine. High levels of IgM antibodies to cpv were first detected seven days after they were vaccinated, and after nine days all the pups had high titres of IgG antibodies to cpv. High levels of IgM antibodies to CDV were detected after nine days and the highest average titres were recorded after 12 days. IgG antibodies to CDV were present from nine days after vaccination.

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