Samples of faeces were taken from 166 healthy domesticated reindeer (Rangifer tarandus tarandus) from three flocks in different reindeer husbandry districts in northern Norway and examined bacteriologically for the presence of Clostridium perfringens. The organism was isolated from 98 (59 per cent) of the reindeer. The isolates were classified into C perfringens toxin types by PKR analysis specific for the genes encoding the four major toxins (α, β, ϵ and ι) and were subclassified by the detection of the genes encoding C perfringens β2-toxin and enterotoxin. All the isolates belonged to C perfringens toxin type A. In addition, 15 of the 98 isolates were PCR-positive for the β2-toxin gene, and two of the isolates had the the gene encoding for enterotoxin.
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