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Identification of Escherichia coli 01 57:H7- strains from pigs with postweaning diarrhoea and amplification of their virulence marker genes by PCR
  1. J. Osek, DVM, PhD1
  1. 1 Department of Microbiology, National Veterinary Research Institute, Partyzantow 57, 24-100 Pulawy, Poland

Abstract

Escherichia coli isolated from pigs with postweaning diarrhoea were examined by PCR for the presence of the 0157 rfb gene responsible for the biosynthesis of E coli 0157 lipopolysaccharide. Among the 372 isolates tested, 38 (10.2 per cent) were of the 0157 serogroup, but none of these possessed the H7 determinant. Further analysis of the E coli 0157 isolates revealed that seven of them had the genes responsible for the production of Shiga toxin 1 and eaeA intimin, four other strains had genes responsible for the production of Shiga toxin 2, and four other strains were positive for the enterohaemolysin gene.

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