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Identification of bluetongue virus serotype 2 (Corsican strain) by reverse-transcriptase PCR reaction analysis of segment 2 of the genome
  1. S. Zientara, DVM, PhD1,
  2. C. Sailleau, BSc1,
  3. G. Dauphin, DVM1,
  4. C. Roquier1,
  5. E. M. Rémond, DVM, PhD1,
  6. F. Lebreton, BSc1,
  7. S. Hammoumi, BSc1,
  8. E. Dubois, PhD1,
  9. C. Agier, BSc1,
  10. G. Merle, BSc1 and
  11. E. Bréard, PhD1
  1. 1 Agence Française de Séurité Sanitaire des Aliments - Alfort, 22 rue Pierre Curie, 94703 Maisons-Alfort, France

Abstract

In October 2000, bluetongue virus was detected on the French island of Corsica. The disease was also reported in Sardinia, Calabria, Sicily and on the Spanish islands of Majorca and Minorca. This paper describes the use of molecular techniques for a rapid identification and serotype determination of serotype 2 of the virus. The nucleotide sequences of segments 2 and 7 of the genome of the Corsican strain were determined and its phylogenetic relationships are described.

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